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Spectrum disposable CellFlo hollow fiber membranes are specially designed for the gentle and
efficient separation of whole cells in microfiltration, bioreactor perfusion and culture harvest
applications. Much like our own circulatory system, CellFlo combines the advantages of tangential
flow microfiltration (0.2 µm and 0.5 µm pores sizes) with larger hollow
fiber flow channels (1 mm ID) to provide gentler efficacious micro-separations
without the risk of cell lysis. Other cell separation technologies have a higher risk of
lysis resulting in ruptured cells and culture harvests contaminated with intracellular
macromolecules. Perfectly suited for continuous cell perfusion and bacterial fermentation,
CellFlo membranes isolate secreted proteins while eliminating spent media containing metabolic
wastes and drawing in fresh nutrient-rich media. Consequently, CellFlo enables cultures to
grow to a higher cell density with higher viability providing as much as a ten-fold increase of
daily production of secreted proteins.
Whether performing a cell perfusion or conducting a simple microfiltration, disposable
CellFlo modules can either be autoclaved or purchased irradiated (irr) for quick sterile
assembly. Spectrum offers disposable CellFlo membrane modules in the full range of MiniKros
and KrosFlo sizes and surface areas for processing volumes ranging from 500 mL
to 1,000 liters. All CellFlo membranes have a 1 mm fiber inner diameter,
available in 0.2 µm and 0.5 µm pore sizes.
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Dimensions:  |
| SA (cm2) | Scale
length | ID (mm) | TL (cm) | L (cm) | EL (cm) |
| 500 - 620 | 1xFL | 18.8 | 27.6 | 22.3 | 20.8 |
| 1500 - 2000 | 1xFL | 31.2 | 27.6 | 22.3 | 20.8 |
| 3200 - 4200 | 2xFL | 31.2 | 51.0 | 46.0 | 44.1 |
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| SA (m2) | Scale
length | ID (mm) | TL (cm) | L (cm) | EL (cm) |
| 0.32 - 1.6 | 1xFL | 70 | 22.9 | 22.9 | 20.0 |
| 1.8 - 3.3 | 2xFL | 70 | 46.7 | 46.0 | 41.5 |
| 2.8 - 5.1 | 3xFL | 70 | 69.1 | 69.1 | 65.0 |
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| Housing: | Clear and Pigmented Polysulfone |
| Connections: | 1/4 HB inlet/outlet ports with FLL filtrate ports
1/2 HB inlet/outlet ports with FLL filtrate ports
3 inch sanitary tri-clover (TC) inlet/outlet ports with 1 1/2 inch sanitary tri-clover(TC) filtrate ports
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| Potting: | Polyurethane |
| Process Volume: | 500 mL to 1,000 liters |
| Surface Area: | 500 cm2 to 4.1 cm2 |
| Membrane | Fiber ID | MWCO |
| Mixed Cellulose Ester (ME) | 1.0 mm | 0.2 µm |
| Polyethersulfone (PES) | 1.0 mm | 0.5 µm, 0.2 µm |
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Advantages of Disposability
- Easier FDA Validation
- Saves Money & Time
- No Cross-Contamination
- Less Down-Time
- Low Shear
- Directly Scalable for Production
- Easy Retrofit for other HF Modules
Applications:
- Cell Concentration, Clarification and Diafiltration
- Lysate Clarification
- VLP and Virus Concentration and Diafiltration
- VLP and Virus Clarification
- Protein Purification, Concentration and Diafiltration
- Nucleic Acid Diafiltration and Concentration
- NanoParticle and Latex Particle Diafiltration and Fractionation
- In situ Conjugation and Fractionation
- Bacteria Concentration and Diafiltration
- Liposome, siRNA Concentration and Diafiltration
- Inclusion body Clarification and Concentration
- Bioreactor cell Perfusion and Media Exchange
RELATED PRODUCTS:
KrosFlo Pilot Plus System
for volumes up to 2,000 L
Learn More >
MiniKros Plus System
for volumes up to 100 liters
Learn More >
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