CellMax Hollow Fiber Bioreactors

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CellMax HF Bioreactors

CelllMax Duo System

CellMax Quad System

CellMax High Flow Quad

Zymax Bioreactors

One CellMax® Bioreactor equals the yield from 100 T-Flasks or 20 Roller Bottles

Hollow fiber cell culture cross-section.

CellMax Hollow Fiber Bioreactors are a low cost alternative to conventional cell culture methods. Unlike traditional cell culture procedures, T-flasks, roller bottles, and two compartment bioreactors, CellMax Hollow Fiber Bioreactors provide a three dimensional space for in vitro cell culture. Hollow fibers are tubular membranes with pore sizes ranging from 10 kD to 0.3µm. Cells grow on and around the large surface area provided by the network of hollow fibers. When perfused with culture media, the hollow fibers allow oxygen and nutrients to be supplied to the cells while metabolic waste products are eliminated. The process increases the accumulation of the cell-secreted growth factors required for optimal growth. Due to the system's efficient delivery of media and removal of waste, the process uses less culture media than other methods. Cultures can be sustained for 6 months or more while maintaining high production yields and titers.

Bioreactor Cartridge Components and Features

Designed for the full range of Cell Culture applications the CellMax Bioreactors feature:

4 Membrane Chemistries including:
- Regenerated Cellulose (RC)
- Polysulfone (PS)
- Polypropylene (PP)
- Polyethylene (PE)
ProNectin® F coated Polyethylene and Polypropylene for enhanced cell adherence.
Surface areas ranging from 100 cm² to 1.6 m²
Animal Derived Component Free
Fully Assembled with Flowpath and Preflushed
Irradiated and Ready to Use
100% Integrity tested

Clave^® Aseptic Access Ports

An important feature of the CellMax Bioreactor is the Clave Aseptic Access Ports which:

Reduce the risk of cell culture contamination
Provide easy access to the ICS (Intra Capillary Space) for monitoring glucose and lactic acid levels.
Provide easy access to the ECS (Extra Capillary Space) for Cell Inoculation or Protein Harvest

Selection of a bioreactor is based on a variety of factors, such as cell line, cell culture application, and performance requirements. The following is a guide to assist in selecting a bioreactor.

CellMax Hollow Fiber Bioreactor Selection Guide:
Adherent cells for collection of secreted products:	RC or PS membranes Cells seeded in the ECS will grow on the surface of the membrane and allow secreted products to concentrate into the ECS media. Depending on the size of the secreted product, select an appropriate MWCO to prevent the protein from permeating through the hollow fiber pore.
Suspension cells for collection of secreted products:	RC membrane The Regenerated Cellulose (RC) membrane is typically preferred. The hydrophilic surface prevents the secreted product from binding to the membrane. This ensures higher titers during harvests and efficient use of the membrane to supply nutrients and eliminate metabolic waste.
Adherent cells for shear stress and gas diffusion:	PP or PE membrane The hydrophobic membrane allows gas to diffuse freely while preventing passage of peptides and proteins, similar to epithelial tight junctions. The large lumen allows you to seed the cells onto the inner capillary surface (inner side of the hollow fiber) and to measure effects of media flow on the adherent cells. These membranes can be coated to enhance cell adhesion and are offered pre-coated with ProNectin F.

Ordering Information: Hollow Fiber Bioreactor Module
		Type	Membrane	MWCO	Area (cm²)	ID (µm)	OD (µm)	ESC (ml)	Price
	430-011	RC	Cellulosic	30 kD	1700	200	216	12	$406
	430-021	RC	Cellulosic	30 kD	10,000	200	216	60	$520
	400-004	RC	Cellulosic	20 kD	16,000	192	200	100	REQUEST
	430-013	PS	Polysulfone	30 kD	1300	200	320	12	$406
	430-023	PS	Polysulfone	30 kD	10,000	200	320	60	$520
	430-010	PS	Polysulfone	10 kD	1700	200	280	12	$406
	430-020	PS	Polysulfone	10 kD	11,000	200	280	60	$520
	410-025	PP	Pre-coated Polypropylene	0.2 µm	100	480	630	1.5	$455
	400-025	PP	Polypropylene	0.2 µm	100	480	630	1.5	$408
	420-007	PP	Pre-coated Polypropylene	0.5 µm	423	480	430	7	$536
	400-007	PP	Polypropylene	0.5 µm	423	480	630	7	$406
	420-015	PE	Pre-coated Polyethylene	0.3 µm	123	380	430	2.3	$503
	400-014	PE	Polyethylene	0.3 µm	123	380	430	2.3	$372
	400-012	PE	Polyethylene	0.3 µm	1,500	380	430	12	$406

Ordering Information: CellMax Accessories
	Part No.	Description	Price
	100-020	Reservoir Cap, 33 mm	$139
	100-025	Reservoir Cap, 38 mm	$139
	100-035	Reservoir Cap, 45 mm	$139